A molecular biologist carries out a PCR reaction and discovers to his disappointment that no PCR amplification takes place. It turns out that the problem is one of the primers, which has the following sequence:

5'-ATAACACTAAATAGCGCTATTTAGTGTTAT-3'

Can you suggest a reason why this primer does not work for PCR?

Question

5'-ATAACACTAAATAGCGCTATTTAGTGTTAT-3'

Can you suggest a reason why this primer does not work for PCR?

Quizplus · Accepted Answer

The Answer of A molecular biologist carries out a PCR reaction and discovers to his disappointment that no PCR amplification takes place. It turns out that the problem is one of the primers, which has the following sequence:

5'-ATAACACTAAATAGCGCTATTTAGTGTTAT-3'

Can you suggest a reason why this primer does not work for PCR?

A Molecular Biologist Carries Out a PCR Reaction and Discovers