When making a complementary DNA (cDNA) library, which enzyme is used to copy mRNA into DNA?
A) DNA ligase
B) DNA polymerase
C) Primase
D) RNA polymerase
E) Reverse transcriptase

Question

Quizplus · Accepted Answer

Reverse transcriptase is the enzyme used to copy mRNA into DNA during cDNA library construction. It is capable of reverse transcribing RNA into cDNA, which can be amplified and cloned to generate a complementary DNA library. DNA ligase is used to join DNA fragments together, DNA polymerase is used to synthesize new DNA strands during PCR, primase is used in DNA replication to synthesize RNA primers, and RNA polymerase copies DNA to RNA during transcription.

When Making a Complementary DNA (CDNA) Library, Which Enzyme Is