Which method of visualizing a protein's location should you use if you want to determine its location in a living cell?
A) a GFP fusion tag
B) immunofluorescence
C) qPCR
D) Gram stain
E) affinity purification

Question

Quizplus · Accepted Answer

A GFP fusion tag allows for real-time visualization of a protein's location within a living cell. Immunofluorescence involves fixing and staining cells and may not accurately reflect the protein's location in its native state. qPCR measures gene expression and does not provide information on protein location. Gram stain is a method of visualizing bacterial cell walls and does not relate to protein location. Affinity purification is a method for isolating and purifying proteins, but does not provide information on their location within a cell.

Which Method of Visualizing a Protein's Location Should You Use