Which technique is NOT used to make a cDNA library?
A) extraction of DNA from the organism you are studying
B) extraction of mRNA from the organism you are studying
C) use of reverse transcriptase to copy RNA into DNA
D) ligation of DNA into a cloning vector
E) insertion of a cloning vector into cells

Question

Quizplus · Accepted Answer

The extraction of DNA would not result in a cDNA library, as cDNA libraries are libraries containing only complementary DNA (cDNA) copies of the mRNA population of a cell or tissue type. Therefore, extraction of mRNA from the organism is required to make a cDNA library.

Which Technique Is NOT Used to Make a CDNA Library