Which of the following would you find in a Sanger sequencing reaction but not in a polymerase chain reaction?
A)DNA template
B)DNA polymerase
C)dNTPs
D)ddNTPs
E)DNA primer

Question

Quizplus · Accepted Answer

Sanger sequencing involves the use of chain-terminating di-deoxynucleotides (ddNTPs), which terminate DNA synthesis when incorporated into a growing DNA strand. These ddNTPs are not used in the polymerase chain reaction (PCR), which uses only normal, deoxynucleotides (dNTPs) for DNA synthesis. Therefore, ddNTPs would be found in a Sanger sequencing reaction but not in PCR.

Which of the Following Would You Find in a Sanger