For which of the following processes can dideoxyribonucleotides be used?
A) to separate different sized DNA fragments
B) to produce cDNA from mRNA
C) to sequence a DNA fragment
D) to visualise DNA expression

Question

Quizplus · Accepted Answer

Dideoxyribonucleotides are used in the Sanger sequencing method to determine the sequence of a DNA fragment. In this method, the dideoxy versions of each nucleotide are added to the reaction, which acts as a chain-terminating nucleotide, resulting in a series of fragments that can be separated by size to determine the sequence. Dideoxyribonucleotides are not used for options A, B, or D.

For Which of the Following Processes Can Dideoxyribonucleotides Be Used