Which of the following processes is an early step in the whole-genome shotgun approach to sequencing?
A) break genomic DNA at random sites
B) map the position of cloned DNA fragments
C) randomly select DNA primers and hybridise these to random positions of chromosomes in preparation for sequencing

Question

Quizplus · Accepted Answer

The whole-genome shotgun approach to sequencing involves breaking genomic DNA at random sites, generating small fragments that can be sequenced and assembled into a complete genome sequence. This is an early step in the process that allows for random sampling of the genome and avoids bias towards certain regions or genes. Mapping the position of cloned DNA fragments and randomly selecting DNA primers are later steps in the process.

Which of the Following Processes Is an Early Step in the Whole-Genome