Gene knockdown is a procedure used to reduce gene expression in cells or organisms so that scientists can investigate gene function. While there are several ways to knock down gene expression, RNAi is one of the most commonly used approaches. Suppose that a research team wants to investigate where a new gene X is expressed in the fruit fly, Drosophila melanogaster. Using RNAi, they obtained the results shown below examining the expression of gene X in four types of tissues.
-Which of the following components could the researchers have used to successfully perform RNAi against gene X?
A) an siRNA molecule that has a complementary sequence to that of gene X
B) a transcription factor that binds to and activates the gene X promoter
C) an enzyme that helps fold the X protein after it is translated

Question

Gene knockdown is a procedure used to reduce gene expression in cells or organisms so that scientists can investigate gene function. While there are several ways to knock down gene expression, RNAi is one of the most commonly used approaches. Suppose that a research team wants to investigate where a new gene X is expressed in the fruit fly, Drosophila melanogaster. Using RNAi, they obtained the results shown below examining the expression of gene X in four types of tissues.
-Which of the following components could the researchers have used to successfully perform RNAi against gene X?
A) an siRNA molecule that has a complementary sequence to that of gene X 
B) a transcription factor that binds to and activates the gene X promoter 
C) an enzyme that helps fold the X protein after it is translated

Quizplus · Accepted Answer

RNAi involves introducing small interfering RNAs (siRNAs) that have a complementary sequence to the target gene, leading to its degradation and reduced expression. Transcription factors and protein-folding enzymes are not involved in RNAi knockdown of gene expression.

Gene Knockdown Is a Procedure Used to Reduce Gene Expression