If a high concentration of a particular nucleotide lacking a hydroxyl group at the 3´ end is added to a PCR reaction,
A) no additional nucleotides will be added to a growing strand containing that nucleotide.
B) strand elongation will proceed as normal.
C) nucleotides will be added only at the 5´ end.
D) T. aquaticus DNA polymerase will become nonfunctional.
E) the primer will be unable to anneal with the DNA template.

Question

If a high concentration of a particular nucleotide lacking a hydroxyl group at the 3´ end is added to a PCR reaction,
A) no additional nucleotides will be added to a growing strand containing that nucleotide.
B) strand elongation will proceed as normal.
C) nucleotides will be added only at the 5´ end.
D) T. aquaticus DNA polymerase will become non­functional.
E) the primer will be unable to anneal with the DNA template.

Quizplus · Accepted Answer

The presence of a nucleotide lacking a hydroxyl group at the 3´ end will prevent the formation of a phosphodiester bond between the nucleotide and the growing strand, effectively terminating elongation of that strand. No additional nucleotides will be added to a growing strand containing this nucleotide.

If a High Concentration of a Particular Nucleotide Lacking a Hydroxyl