What happens when substrate concentration is much higher than Km?
A) Equilibrium is reached
B) Km is increased
C) The rate is equal to Kcat [E]
D) The rate is linear
E) The reaction cannot continues

Question

Quizplus · Accepted Answer

When substrate concentration is much higher than Km, the rate of the reaction is limited only by the rate at which the enzyme can convert the substrate to product, which is determined by Kcat (turnover number) and the concentration of enzyme ([E]). At saturation, all the enzyme molecules are bound to the substrate and the reaction rate becomes constant and equal to Kcat [E].

What Happens When Substrate Concentration Is Much Higher Than Km