Which one of the following properties would make a plasmid an ineffective vehicle?
A) very small size
B) multiple copies are incorporated per cell
C) codes for the protein required for tetracycline resistance
D) contains three sites for the restriction endonuclease to be used

Question

Quizplus · Accepted Answer

Plasmids are effective vehicles for gene cloning because they can easily incorporate foreign DNA. However, if a plasmid contains multiple sites for the restriction enzyme being used, it could be cut into several pieces, making it difficult to insert the desired DNA fragment and recover the intact plasmid. This would make it an ineffective vehicle for gene cloning.

Which One of the Following Properties Would Make a Plasmid