Which of the following techniques can be used to screen the general population for a single base-pair mutation in disease-causing gene?
A) RFLP linkage analysis
B) Allele specific oligonucleotide (ASO) analysis
C) Fluorescence in situ hybridization (FISH)
D) Multiplex PCR

Question

Quizplus · Accepted Answer

Allele specific oligonucleotide (ASO) analysis is a technique that can be used to screen the general population for a single base-pair mutation in a disease-causing gene. This technique involves designing oligonucleotides that are specific to the wild-type and mutant alleles of the gene, and using these oligonucleotides to detect the presence or absence of the mutation in DNA samples. RFLP linkage analysis, FISH, and multiplex PCR are not suitable for screening the general population for a single base-pair mutation.

Which of the Following Techniques Can Be Used to Screen