Isolation and identification of a disease-causing gene by chromosome walking requires all of the following EXCEPT
A) Genomic library
B) DNA sequence analysis
C) Comparison of the normal and mutant genes
D) Prior knowledge of the protein product
E) Overlapping genomic clones

Question

Quizplus · Accepted Answer

Prior knowledge of the protein product is not necessary for the process of chromosome walking, as this technique relies on the physical linkage of DNA sequences on a chromosome rather than knowledge of the gene's function or protein product. The other options listed are all critical components of chromosome walking.

Isolation and Identification of a Disease-Causing Gene by Chromosome Walking