Which of the following statements about N-glycosylation of proteins is not true:
A) The oligosaccharide contains both mannose (Man) and N-acetyl- glucosamine (GlcNac) residues.
B) The oligosaccharide is built up on the protein beginning with attachments of GlcNac to asparagine and subsequent additions of either GlcNac or Man residues from the corresponding UDP sugars.
C) The transfer is made from a lipid intermediate called dolichol.
D) Transfer occurs within the endoplasmic reticulum.
E) The biosynthesis of dolichol-PP-oligosaccharide is inhibited by tunicamycin.

Question

Quizplus · Accepted Answer

The oligosaccharide is initially assembled on a dolichol phosphate lipid carrier before being transferred en bloc to the asparagine residue of the protein. The process does not involve building the oligosaccharide directly on the protein through sequential additions of sugars to asparagine.

Which of the Following Statements About N-Glycosylation of Proteins Is