Edman degradation refers to
A) a metabolic pathway for catabolism of proteins.
B) a chemical method for determination of the N-terminal amino acid sequence of proteins and polypeptides.
C) an enzymatic method for determination of the C-terminal sequence of proteins and polypeptides.
D) a chemical method for determination of the C-terminal sequence of proteins and polypeptides.
E) None of the above statements correctly refer to the Edman degradation.

Question

Quizplus · Accepted Answer

Edman degradation is a chemical method used to determine the N-terminal amino acid sequence of proteins and polypeptides. It involves the sequential removal of one amino acid from the N-terminus of the protein and its identification by chromatographic or spectroscopic analysis. This method is widely used in protein sequencing and characterization.

Edman Degradation Refers to