Refer to the figure and table below.
A researcher treated mouse kidney cells growing in culture so that they took up a fluorescent dye into their cytosols without losing viability.(This dye undergoes a change in fluorescence intensity upon binding calcium ions.) The treated cells were then incubated with and without a compound thought to act as a signaling molecule, and the cells were observed for changes in fluorescence.The researcher hypothesizes that the signaling molecule functions according to the pathway shown in the diagram above.As a next step, the researcher wants to acquire evidence that IP3 is involved in this pathway.Which procedure will provide needed evidence about IP3 as the entire experiment is repeated?
A) Using mouse kidney cells containing a mutation that knocks out GTP binding by G protein
B) Using mouse kidney cells containing a mutation that knocks out phospholipase C
C) Adding a calcium channel inhibitor during all trials
D) Replacing the signaling molecule with an agonist molecule
E) Replacing the signaling molecule with IP3
Correct Answer:
Verified
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