What method is used to release the N-linked glycan groups from the membrane-associated glycoconjugates shown in 1 in the figure below?
A) enzymatic cleavage using PNGaseF
B) chemical cleavage using a -elimination reaction
C) chemical cleavage using a Schiff base intermediate
D) enzymatic cleavage using -galactosidase

Question

What method is used to release the N-linked glycan groups from the membrane-associated glycoconjugates shown in 1 in the figure below? 
A) enzymatic cleavage using PNGaseF 
B) chemical cleavage using a -elimination reaction 
C) chemical cleavage using a Schiff base intermediate 
D) enzymatic cleavage using -galactosidase

Quizplus · Accepted Answer

Enzymatic cleavage using PNGaseF is the method used to release the N-linked glycan groups from the membrane-associated glycoconjugates shown in 1. PNGaseF is an enzyme that specifically cleaves the N-glycosidic bond between the asparagine residue and the N-linked glycan. This method is commonly used in glycoprotein analysis and glycan profiling. Chemical cleavage using a 11ea8d43_ab9b_2cfe_b445_ab84204d5854_TB6697_11_TB6697_11-elimination reaction, chemical cleavage using a Schiff base intermediate, and enzymatic cleavage using 11ea8d43_ab9b_2cff_b445_819a5ee8c740_TB6697_11_TB6697_11-galactosidase are not appropriate methods for releasing N-linked glycans.

What Method Is Used to Release the N-Linked Glycan Groups