A 3-kb supercoiled circular plasmid contains the restriction sites shown below.N and X represent NheI and XbaI sites,respectively;A through F mark six points on the plasmid.A preparation of plasmid DNA was separately digested to completion with XbaI alone,NheI alone,and a mixture of the two enzymes.The products were separated by agarose gel electrophoresis.Draw the predicted appearance of the gel,assuming that you ran the following in the various lanes.
Lane M (size markers)-a mixture of known markers increasing in size from 1 kb to 6 kb,in steps of 1 kb
Lane 1-sample from the original plasmid DNA preparation
Lane 2-products of complete digestion with XbaI alone
Lane 3-products of complete digestion with NheI alone
Lane 4-products of complete digestion with XbaI plus NheI

Question

A 3-kb supercoiled circular plasmid contains the restriction sites shown below.N and X represent NheI and XbaI sites,respectively;A through F mark six points on the plasmid.A preparation of plasmid DNA was separately digested to completion with XbaI alone,NheI alone,and a mixture of the two enzymes.The products were separated by agarose gel electrophoresis.Draw the predicted appearance of the gel,assuming that you ran the following in the various lanes.  
Lane M (size markers)-a mixture of known markers increasing in size from 1 kb to 6 kb,in steps of 1 kb
Lane 1-sample from the original plasmid DNA preparation
Lane 2-products of complete digestion with XbaI alone
Lane 3-products of complete digestion with NheI alone
Lane 4-products of complete digestion with XbaI plus NheI

Quizplus · Accepted Answer

The Answer of A 3-kb supercoiled circular plasmid contains the restriction sites shown below.N and X represent NheI and XbaI sites,respectively;A through F mark six points on the plasmid.A preparation of plasmid DNA was separately digested to completion with XbaI alone,NheI alone,and a mixture of the two enzymes.The products were separated by agarose gel electrophoresis.Draw the predicted appearance of the gel,assuming that you ran the following in the various lanes.  
Lane M (size markers)-a mixture of known markers increasing in size from 1 kb to 6 kb,in steps of 1 kb
Lane 1-sample from the original plasmid DNA preparation
Lane 2-products of complete digestion with XbaI alone
Lane 3-products of complete digestion with NheI alone
Lane 4-products of complete digestion with XbaI plus NheI

A 3-Kb Supercoiled Circular Plasmid Contains the Restriction Sites Shown