When EcoRI recognizes the sequence G-A-A-T-T-C it cuts a strand between the G and A on the double strand.What is the sequence of the sticky end on one of the strands of the DNA strand shown?
5' 3'
-G-A-A-T-T-C-
-C-T-T-A-A-G-
3' 5'
A)-C-T-T-A-A
B)A-A-T-T-C-
C)G-A-A-T-C
D)Both A and B are correct.
E)All of these are correct.

Question

Quizplus · Accepted Answer

EcoRI cuts between G and A, creating overhangs. The sequence on one strand is 5'-AATTC and on the complementary strand is 3'-CTTAA, which corresponds to options A and B.

When EcoRI Recognizes the Sequence G-A-A-T-T-C It Cuts a Strand