What is the correct sequence for a Gram stain?
A)Gram's iodine, crystal violet, alcohol, safranin
B)crystal violet, safranin, alcohol, Gram's iodine
C)crystal violet, Gram's iodine, alcohol, safranin
D)safranin, crystal violet, Gram's iodine, alcohol
E)alcohol, crystal violet, safranin, Gram's iodine
04)13 Summarize how gram-positive and gram-negative cells differ in their reactions.

Question

Quizplus · Accepted Answer

The correct sequence for a Gram stain is crystal violet, Gram's iodine, alcohol, safranin. Gram-positive cells will retain the crystal violet stain, while gram-negative cells will lose it after being washed with alcohol. Gram-negative cells will then take up the counterstain, safranin, while gram-positive cells will not. This difference in staining is due to differences in the cell wall composition between the two types of bacteria. Gram-positive cells have a thick peptidoglycan layer in their cell walls, while gram-negative cells have a thinner peptidoglycan layer and an outer membrane containing lipopolysaccharides.

What Is the Correct Sequence for a Gram Stain