Which of the following reagents reacts with crystal violet as the mordant?
A)Crystal violet
B)Gram's iodine
C)95% ethyl alcohol
D)Safranin

Question

Quizplus · Accepted Answer

Gram's iodine is the mordant that reacts with crystal violet in the Gram staining technique. Crystal violet first stains all the cells in the sample and then Gram's iodine forms a complex with it, making the stain insoluble in water. This allows the stain to persist during the washing step with alcohol and differentiate between Gram-positive and Gram-negative bacteria. 95% ethyl alcohol is used as a decolorizing agent, and safranin is used as a counterstain. Therefore, options A, C, and D are incorrect.

Which of the Following Reagents Reacts with Crystal Violet as the Mordant