Which of the following are required for PCR?
A) knowing a protein's amino acid sequence
B) primers complementary to the entire target DNA sequence
C) the four nucleotides in the proportions of the target DNA sequence
D) Taq2 RNA polymerase
E) knowing part of the target DNA sequence that you want to amplify

Question

Quizplus · Accepted Answer

PCR requires short nucleotide primers that are complementary to the target DNA sequence. Therefore, knowing part of the target DNA sequence that you want to amplify is a requirement for PCR. A protein's amino acid sequence and Taq2 RNA polymerase are not necessary components of PCR. Additionally, while the proportions of the four nucleotides are important, they do not need to be in the exact proportions of the target DNA sequence as long as they are present in sufficient quantities for the reaction to proceed.

Which of the Following Are Required for PCR