In a FISH experiment,what would happen if unbound probe was not washed off?
A)Nothing-it's not necessary to wash off the unbound probe.
B)You would get false-positive results in different areas where the probe hadn't actually bound,but it was still sitting there and lighting up.
C)Your FISH would be floating at the top of the tank due to the toxicity of the probe building up within them.
D)Nothing-the target nucleotide sequences are labeled,not the probe.Therefore,excess unbound probe wouldn't matter for the experiment.

Question

Quizplus · Accepted Answer

If unbound probe is not washed off, it can bind to non-target areas and produce false-positive results. This is why it is important to wash off unbound probe after hybridization in FISH experiments. Choice A and D are incorrect because washing off unbound probe is necessary in FISH experiments. Choice C is incorrect because probes do not have a toxic effect and excess unbound probe would not cause FISH to float at the top of the tank.

In a FISH Experiment,what Would Happen If Unbound Probe Was