When compared with non-cancerous cells of the same tissue,cancer cells exhibit a number of changes in gene transcription.To identify genes that are differentially expressed in cancer cells versus non-cancer cells,one could perform
A)subtractive hybridization.
B)a northern blot.
C)RFLP analysis.
D)PCR.

Question

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Subtractive hybridization is a technique used to isolate differentially expressed genes. It is based on the principle that if two DNA samples are hybridized, the more abundant mRNA sequences will be represented in a greater amount by the corresponding cDNA. By subtracting the cDNA from the non-cancerous cells (control) from the cDNA of cancer cells (test), the differentially expressed genes can be isolated. Northern blot, RFLP analysis, and PCR can also be used to study gene expression changes, but subtractive hybridization is the most appropriate choice for identifying gene expression changes on a genome-wide scale.

When Compared with Non-Cancerous Cells of the Same Tissue,cancer Cells