A polymerase chain reaction (PCR)involves three steps.The correct order of those steps is
A)denaturation,annealing of primers,and then primer extension.
B)annealing of primers,denaturation,and then primer extension.
C)primer extension,annealing of primers,and then denaturation.

Question

Quizplus · Accepted Answer

In a PCR, the first step is denaturation, where the double-stranded DNA template is heated to separate the strands. The second step is annealing of primers, where the temperature is lowered to allow the primers to bind to the complementary sequences on either side of the target gene. The third step is primer extension, where a heat-stable DNA polymerase extends the primers along the template to create new copies of the target DNA sequence. Therefore, the correct order of steps is denaturation, annealing of primers, and then primer extension, which corresponds to choice A.

A Polymerase Chain Reaction (PCR)involves Three Steps