In your cell biology laboratory class,you are given a sample of blood.Which of the following results are NOT possible from your analysis of this blood using flow cytometry?
A)isolation of the largest and most dense cells from the smaller blood cells
B)measurement of the amount of DNA in white blood cells
C)relative quantitation of the sizes and shapes of the cells in the blood sample
D)after the addition of fluorescent antibodies specific to T cells,the analysis of T cell abundance and size relative to other cells in the sample

Question

Quizplus · Accepted Answer

Flow cytometry is not capable of isolating cells based on size and density. It can only analyze cells based on their physical and chemical characteristics, such as size, shape, surface markers, and fluorescence. Cell sorting based on size and density can be achieved using other techniques such as density gradient centrifugation or cell sorting based on morphology, but not flow cytometry.

Answer: C, D
 Flow cytometry can perform relative quantitation of the sizes and shapes of cells in a sample, as well as analyze the abundance and size of specific cell types using fluorescent antibodies. It can also measure the amount of DNA in cells using staining with DNA-specific dyes. Therefore, options C and D are possible results of flow cytometry analysis of blood samples.

In Your Cell Biology Laboratory Class,you Are Given a Sample