In affinity chromatography
A) there is nonspecific binding of proteins to column material
B) only minor purifications can be obtained
C) the mobile phase is always pure water
D) the ligand is alwayse specific for one type of protein to be bound
E) there can be molecule specific ligands or group specific ligands

Question

Quizplus · Accepted Answer

In affinity chromatography, there can be molecule specific ligands or group specific ligands, allowing for targeted binding of specific proteins. This allows for significant purification to be obtained. Nonspecific binding can be minimized through proper column material selection and the mobile phase is not necessarily pure water, but can include buffers or other solvents.

In Affinity Chromatography