Complementation experiments are used to determine if two observed mutations that are associated with the same trait are actually in the same gene.

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Complementation experiments involve crossing two individuals with different mutations and observing the offspring. If the mutations are in different genes, the offspring will show the phenotype associated with both mutations. However, if the mutations are in the same gene, the offspring will show a phenotype that is similar to only one of the mutations. Therefore, complementation experiments can be used to determine if two observed mutations that are associated with the same trait are actually in the same gene.

Complementation Experiments Are Used to Determine If Two Observed Mutations