Place the steps of constructing a genomic library in order.
I.Digest phage with restriction enzyme.
II.Lyse cells of interest and precipitate DNA.
III.Introduce constructs via transformation into competent E.coli cells.
IV.Perform ligation reaction with genome fragments and phage.
V.Digest genomic DNA with restriction enzyme.
A)V,IV,I,III.II.
B)I,II,III,IV,V.
C)II,I,III,V,IV.
D)II,V,I,IV,III.
E)IV,II,III,I,V.

Question

Quizplus · Accepted Answer

The correct sequence for constructing a genomic library involves: (II) lysing cells and precipitating DNA to obtain the genomic material, (V) digesting the genomic DNA with a restriction enzyme to create fragments, (I) similarly digesting a vector (like a phage) with the same restriction enzyme to prepare it for the insertion of genomic fragments, (IV) performing a ligation reaction to insert the genomic fragments into the vector, and (III) introducing the constructs into competent E. coli cells via transformation for replication and storage.

Place the Steps of Constructing a Genomic Library in Order