Nested polymerase chain reaction (PCR)is very sensitive and specific because:
A) It uses more than one DNA polymerase to make three to four times the amount of PCR products than standard PCR.
B) The assay itself basically serves as a form of internal control and ensures specificity.
C) The assay will act as a form of external control, ensuring specificity and sensitivity.
D) The fluorochrome that is attached to the primers ensures specificity of the nucleotides that are attached to the template.

Question

Quizplus · Accepted Answer

The assay itself basically serves as a form of internal control and ensures specificity.Nested PCR consists of two different,consecutive PCR assays.The first reaction is a standard PCR assay using one set of PCR primers.The amplicon produced from this first reaction is then used as the target in a subsequent PCR assay.The second primer pair is complementary to an internal region of the amplicon derived from the first PCR assay.Amplicon will only be synthesized during this second PCR assay if amplicon was produced in the first reaction.

Nested Polymerase Chain Reaction (PCR)is Very Sensitive and Specific Because