In a hybridization reaction,the pH affects:
A) The probe's nucleic acids so that they do not lose their hydrogens
B) The breakdown of the oligonucleotide pieces
C) The ability of the mismatches between the probe and the target to duplex
D) The stability of double-stranded nucleic acid molecules in solution

Question

Quizplus · Accepted Answer

pH affects the stability of double-stranded nucleic acid molecules in solution.An alkaline pH promotes dissociation of double-stranded molecules,whereas acidic pH solutions can depurinate probes and target nucleic acid molecules.

In a Hybridization Reaction,the PH Affects