Which is appropriate for initial rate method experiments to analyze an enzyme-catalyzed reaction?
A)Substrate concentration is constant and the rate is measured at different concentrations of enzyme.
B)Enzyme concentration is constant and the rate is measured at different substrate concentrations.
C)The substrate and enzyme concentrations are varied inversely with respect to one another and the rate is measured.
D)Both enzyme and substrate concentrations are constant.The temperature is varied and the rate is measured.
E)All of the above.

Question

Quizplus · Accepted Answer

The initial rate method involves measuring the initial rate of the reaction at varying concentrations of either the substrate or the enzyme, while keeping the concentration of the other constant. This allows for the determination of the enzyme's kinetic parameters, including the Michaelis-Menten constant (Km) and the maximum velocity (Vmax), which provide information on the enzyme-substrate interaction and the efficiency of the catalytic process. Option B is the appropriate choice for initial rate experiments, as it keeps the concentration of the enzyme constant and measures the rate at different substrate concentrations.

Which Is Appropriate for Initial Rate Method Experiments to Analyze