A mixture of two proteins with the same pI and molecular weights (MW)of 10,000 and 15,000 daltons,respectively,are applied to a gel filtration column.What happens during elution?
A)Both elute together.
B)The one with MW of 10,000 elutes first.
C)The one with MW of 15,000 elutes first.
D)More information is needed about the specificity of the column matrix to tell what happens.

Question

Quizplus · Accepted Answer

Gel filtration chromatography separates molecules based on size, with larger molecules eluting first because they cannot enter the pores of the gel matrix as easily as smaller molecules.

A Mixture of Two Proteins with the Same PI and Molecular