You are studying the functions associated with adiponectin by utilizing site-directed mutagenesis of an adiponectin cDNA followed by expression of the cDNA in transgenic mice. One particular mutation abolishes nearly 100% of the activity of the protein but does not affect the level of protein found in the circulation of the test mice. This mutation most likely blocks which of the following modifications that is required for adiponectin function?
A) acetylation
B) glycosylation
C) phosphorylation
D) prenylation
E) ubiquitination

Question

Quizplus · Accepted Answer

Glycosylation is a post-translational modification important for the proper folding, stability, and function of many proteins, including adiponectin. The mutation likely disrupts glycosylation, affecting the protein's activity without altering its circulating levels.  Adiponectin contains a C-terminal globular domain that allows for a homotrimeric association of the protein forming the functional structure of the protein. The hormone forms complex structures such that it can be found as a trimer, hexamer, and as the high-molecular-weight oligomer. In addition to the complex structure, adiponectin is glycosylated, a modification that is essential to its activity.

You Are Studying the Functions Associated with Adiponectin by Utilizing