Kawakubo's group created a glycoprotein with a terminal NAG (i.e.,a protein with NAG attached to its end).Their hypothesis is that the terminal NAG-and not the protein component-is responsible for the damage to the cell wall in H.pylori.What would be the most appropriate control for testing this hypothesis?
A)Grow H.pylori in a test tube (in vitro)with no glycoprotein.
B)Destroy the H.pylori by exposing them to a hypotonic solution.Then add the glycoprotein and observe.
C)Expose other species of bacteria to the glycoprotein.
D)Grow H.pylori in a test tube with glycoprotein that has its terminal NAG removed.

Question

Quizplus · Accepted Answer

The best control for testing the hypothesis is to grow H.pylori in a test tube with glycoprotein that has its terminal NAG removed. This will help in determining if the glycoprotein or the terminal NAG is responsible for the damage to the cell wall in H.pylori. A) is not an appropriate control as it does not include the glycoprotein at all. B) is not an appropriate control as it involves destroying the H.pylori before adding the glycoprotein. C) is not an appropriate control as it involves testing other species of bacteria, which may not be relevant to the hypothesis.

Kawakubo's Group Created a Glycoprotein with a Terminal NAG (I