If you wanted precisely detect the change in mRNA levels responsible for directing the manufacture of a particular protein in a section of the rat brain after treatment with a drug, which technique would you use?
A) Functional MRI
B) In situ hybridization (ISH)
C) Computerized tomography
D) SPECT

Question

Quizplus · Accepted Answer

In situ hybridization (ISH) is a technique that allows for the visualization and detection of specific mRNA transcripts within cells or tissues. This technique is useful for detecting changes in mRNA levels in response to various treatments, such as drug treatments. Therefore, if one wanted to precisely detect the change in mRNA levels responsible for directing the manufacture of a particular protein in a section of the rat brain after treatment with a drug, ISH would be the best technique to use. Functional MRI, computerized tomography, and SPECT are imaging techniques that do not directly measure mRNA levels and would not be suitable for this purpose.

If You Wanted Precisely Detect the Change in MRNA Levels