In RNA interference, expression of a gene is most effectively inhibited by injection of
A) antisense RNA complementary to its mRNA.
B) sense RNA complementary to its mRNA.
C) double-stranded DNA.
D) double-stranded RNA.

Question

Quizplus · Accepted Answer

In RNA interference, double-stranded RNA is most effective in inhibiting the expression of a gene. When double-stranded RNA is introduced into a cell, it is cleaved by an enzyme called Dicer, producing small interfering RNA (siRNA) molecules. These siRNAs then bind to the mRNA corresponding to the targeted gene, leading to its degradation and subsequent inhibition of gene expression. Antisense RNA and sense RNA can also be used in gene regulation, but they are not as effective as double-stranded RNA in RNA interference. Double-stranded DNA is not typically used in RNA interference as it cannot be directly processed by Dicer into siRNAs.

In RNA Interference, Expression of a Gene Is Most Effectively