The key elements of a PCR reaction are ____.
A) the four nucleoside triphosphates, DNA primase, DNA polymerase, and an agarose gel
B) a pair of primers, the four nucleoside triphosphates, DNA primase, and DNA polymerase
C) DNA with the target sequence to be amplified, the four nucleoside triphosphates, DNA primase, and DNA polymerase
D) DNA with the target sequence to be amplified, a pair of primers, the four nucleoside triphosphates, and DNA primase
E) DNA with the target sequence to be amplified, a pair of primers, the four nucleoside triphosphates, and DNA polymerase

Question

Quizplus · Accepted Answer

The key elements of a PCR reaction include DNA with the target sequence to be amplified, a pair of primers to initiate DNA synthesis, the four nucleoside triphosphates (dATP, dCTP, dGTP, dTTP) as the building blocks of DNA, and DNA polymerase to synthesize the new DNA strands. DNA primase and agarose gel are not required for the PCR reaction itself, although agarose gel electrophoresis may be used to analyze the PCR products.

The Key Elements of a PCR Reaction Are ____