In E. coli, a mutation in a gene called dnaB prevents the helicase from binding at the origin of replication. Which of the following events would you expect to occur as a result of this mutation?
A) Additional proofreading will occur.
B) No replication fork will be formed.
C) Replication will occur via RNA polymerase alone.
D) Replication will require a DNA template from another source.

Question

Quizplus · Accepted Answer

Helicase is essential for unwinding the DNA double helix at the origin of replication, allowing the replication machinery to access the single-stranded DNA. Without the action of helicase, the replication fork, where the DNA is unwound and replication occurs, cannot be formed.

In E Coli, a Mutation in a Gene Called DnaB Prevents