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Affinity chromatography is a method that can be used to purify cell-surface receptors, while they retain their hormone-binding ability. A ligand (hormone) for a receptor of interest is chemically linked to polystyrene beads. A solubilized preparation of membrane proteins is passed over a column containing these beads. Only the receptor binds to the beads.
-This method of affinity chromatography would be expected to collect which of the following?
A) molecules of the hormone
B) molecules of purified receptor
C) G proteins
D) assorted membrane proteins
E) hormone-receptor complexes

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Quizplus · Accepted Answer

The method of affinity chromatography is designed to purify cell-surface receptors while retaining their hormone-binding ability. The ligand-hormone is chemically linked to polystyrene beads and only the receptor binds to the beads. Therefore, this method is expected to collect molecules of the purified receptor.

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