Successful insertion of a DNA fragment into the polylinker region of pUC19 is detected by
A) autoradiography.
B) production of the enzyme β-galactosidase.
C) absence of the enzyme β-galactosidase.
D) the increased size of the plasmid.
E) none of the above.

Question

Quizplus · Accepted Answer

The insertion of a DNA fragment into the polylinker region of pUC19 disrupts the lacZ gene, preventing the production of β-galactosidase, which can be detected by the absence of blue color in X-gal assays.

Successful Insertion of a DNA Fragment into the Polylinker Region