In selecting recombinant bacteria, cells are chosen that are resistant to a specific antibiotic. How are the bacteria made resistant?
A) Resistance is activated when the cells are provided with the antibiotic
B) Resistance is activated by the recombination event.
C) The antibiotic resistance gene is encoded in the vector.
D) They are pre- selected for the experiment on this basis.
E) The antibiotic resistance gene is encoded on the donor insert.

Question

Quizplus · Accepted Answer

The antibiotic resistance gene is typically encoded in the vector used for cloning. This allows for the selection of bacteria that have successfully taken up the vector by growing them on media containing the antibiotic; only those bacteria with the vector, and thus the resistance gene, will survive.

In Selecting Recombinant Bacteria, Cells Are Chosen That Are Resistant