Which of the following strains will be useful in demonstrating trans- regulation? In the genotypes listed below,the genes on the chromosome are on the left of the slash (/)symbol and the genes F' episome are on the right.

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In this genotype, the lacI gene on the chromosome is wild type (lacI+), while the lacI gene on the F' episome is a mutant (lacI-). This means that the lacI gene on the chromosome will be able to produce a functional LacI repressor, while the lacI gene on the F' episome will not. As a result, the lac operon on the chromosome will be repressed, while the lac operon on the F' episome will be derepressed. This will allow us to observe the effects of trans-regulation, as the expression of the lac operon on the chromosome will be dependent on the presence of the functional LacI repressor on the F' episome.

Which of the Following Strains Will Be Useful in Demonstrating