Which of the following processes is an early step in the whole-genome shotgun approach to sequencing?
A) break genomic DNA at random sites
B) map the position of cloned DNA fragments
C) randomly select DNA primers and hybridize these to random positions of chromosomes in preparation for sequencing

Question

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The whole-genome shotgun approach to sequencing involves breaking genomic DNA at random sites, generating overlapping fragments of different sizes, and then sequencing these fragments. This is an early step in the process of generating a complete genome sequence. Options B and C are not early steps in this approach. Mapping the position of cloned DNA fragments is done after the sequencing is complete to assemble the final genome sequence, while randomly selecting DNA primers is not specific to the whole-genome shotgun approach and can be used in other sequencing methods as well.

Which of the Following Processes Is an Early Step in the Whole-Genome