Why is it so important to be able to amplify DNA fragments when studying genes?
A) Before amplification, DNA fragments are likely to bind to RNA and would no longer be able to be analyzed.
B) A gene may represent only a millionth of the cell's DNA.
C) Restriction enzymes (endonucleases) cut DNA into fragments that are too small.
D) A clone requires multiple copies of each gene per clone.

Question

Quizplus · Accepted Answer

Amplification allows for the production of multiple copies of a DNA fragment, which is important when studying genes because a gene may represent only a small fraction of the total DNA present in a cell. Without amplification, it would be difficult to study and analyze the gene of interest.

Answer: D
 In gene cloning, it is important to have multiple copies of each gene of interest. Amplification allows for the production of multiple copies of a DNA fragment, which can then be cloned and used for further study.

Why Is It So Important to Be Able to Amplify