What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
I.Transform bacteria with a recombinant DNA molecule.
II.Cut the plasmid DNA using restriction enzymes (endonucleases).
III.Extract plasmid DNA from bacterial cells.
IV.Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments.
V.Use ligase to seal plasmid DNA to non-plasmid DNA.
A) II, III, V, IV, I
B) III, II, IV, V, I
C) III, IV, V, I, II
D) IV, V, I, II, III

Question

Quizplus · Accepted Answer

The plasmid DNA must be cut using restriction enzymes (II) before it can be joined to foreign DNA fragments through hydrogen bonding (IV) and sealed with ligase (V). Plasmid DNA is then extracted from the bacterial cells (III) and transformed into new bacterial cells to produce recombinant bacteria (I).

What Is the Most Logical Sequence of Steps for Splicing