To make an adequate library for sequencing prokaryotic genes, a vector should hold inserts in the range of
A) 10- 50 kbp.
B) 0.5- l kbp.
C) 50- 100 kbp.
D) 2- 10 kbp.

Question

Quizplus · Accepted Answer

The ideal insert size for a prokaryotic gene library is in the range of 2-10 kbp. This is because most prokaryotic genes are relatively small, with an average size of about 1 kbp. A vector with an insert size of 2-10 kbp will allow for efficient cloning of full-length genes without introducing excessive vector sequences or leaving out important regulatory regions. Additionally, vectors with smaller inserts are easier to handle and manipulate, and are less likely to cause problems during sequencing or downstream applications.

To Make an Adequate Library for Sequencing Prokaryotic Genes, a Vector