Which molecular technique requires the complementary strand of a nucleotide sequence of interest as a probe to identify its presence?
A) FISH
B) AFLP
C) MLST
D) FAME

Question

Quizplus · Accepted Answer

FISH (Fluorescence in situ hybridization) requires the use of a complementary nucleotide probe that binds to the target sequence being analyzed. By using fluorescently labeled probes, the location and abundance of the target sequence can be visualized using a fluorescence microscope. AFLP (Amplified Fragment Length Polymorphism), MLST (Multi-locus sequence typing), and FAME (Fatty Acid Methyl Ester) are not techniques that involve the use of a nucleotide probe.

Which Molecular Technique Requires the Complementary Strand of a Nucleotide