Which of the following would be an appropriate sequence of temperatures for PCR?
A) 94°C, 37°C, 55°C
B) 94°C, 55°C, 37°C
C) 94°C, 65°C, 72°C
D) 72°C, 65°C, 94°C
E) 65°C, 72°C, 94°C

Name: Which of the following would be an appropriate sequence of temperatures for PCR? A) 94°C, 37°C, 55°C B) 94°C, 55°C, 37°C C) 94°C, 65°C, 72°C D) 72°C, 65°C, 94°C E) 65°C, 72°C, 94°C
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Question

Quizplus · Accepted Answer

PCR involves denaturation at 94°C, annealing at around 55-65°C, and extension at 72°C.

Which of the Following Would Be an Appropriate Sequence of Temperatures