What is one difference between using restriction endonucleases and mechanical shearing of DNA?
A)Restriction endonucleases cut DNA at known specific sites while shearing occurs at random sites.
B)Restriction endonucleases cut DNA at random sites while shearing occurs at known specific sites.
C)DNA cut with restriction enzymes can be used for cloning and DNA broken by shearing cannot.
D)Restriction enzymes produce larger fragments on average than shearing.

Question

Quizplus · Accepted Answer

Restriction endonucleases are enzymes that recognize specific sequences of DNA and cut the DNA at those sites, allowing for precise manipulation of DNA fragments. Mechanical shearing, on the other hand, breaks DNA randomly, without sequence specificity. This makes restriction endonucleases useful for tasks requiring specific DNA fragments, such as cloning or mapping.

What Is One Difference Between Using Restriction Endonucleases and Mechanical